Peer-reviewed journal publications 

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JJ Kim*, P Chan*, J Vlassakis, A Geldert, & AE Herr. "Microparticle delivery of protein markers for single-cell western blotting from microwells." Small, 2018, accepted. *Equal contributors

E Sinkala*, E Rosàs-Canyelles*, & AE Herr. "Single-cell mobility shift electrophoresis reports protein localization to the cell membrane." Analyst, 2018, accepted. *Equal contributors

E Rosàs-Canyelles, T Dai, S Li, & AE Herr. "Mouse-to-mouse variation in maturation heterogeneity of smooth muscle cells." Lab Chip, 2018, 18(13):1875-1883. doi: 10.1039/c8lc00216a. PMID: 29796562.

C-C Kang, T. Ward, J. Bockhorn, C. Schiffman, H. Huang, M. Pegram, & AE Herr. "Electrophoretic cytopathology resolves ERBB2 forms with single-cell resolution." npj Precision Oncology, 2018, 2:10. doi: 10.1038/s41698-018-0052-3. PMID: 29872719.

K Yamauchi*, AM Tentori*, & AE Herr. "Arrayed isoelectric focusing using photopatterned multi-domain hydrogels." Electrophoresis, 2018, 39(8):1040-1047. doi: 10.1002/elps.201700386. PMID: 29385243 *Equal contributors

Y Zhang, P Chan & AE Herr. "Rapid capture and release of nucleic acids through a reversible photo-cycloaddition reaction in a psoralen-functionalized hydrogel." Angewandte Chemie, 2018, 57(9):2357-2361. doi: 10.1002/anie.201711441. PMID: 29316080.

J-MG Lin, C-C Kang, Y Zhou, H Huang, AE Herr, & S Kumar. "Linking invasive motility to protein expression in single tumor cells." Lab Chip, 2018, 18(2):371-384. doi: 10.1039/c7lc01008g. PMID: 29299576

Q Pan & AE Herr. "Geometry-induced injection dispersion in single-cell protein electrophoresis." Analytica Chimica Acta , 2017, 1000:214-222. doi: 10.1016/j.aca.2017.11.049. PMID: 29289313

J Vlassakis & AE Herr. "Joule Heating-induced dispersion in open microfluidic electrophoretic cytometry." Analytical Chemistry, 2017, 89(23):12787-12796. doi: 10.1021/acs.analchem.7b03096. PMID: 29110464

EJ Su & AE Herr. "Electrophoretic cytometry of adherent cells." Lab Chip, 2017, 17(24):4312-4323. doi: 10.1039/C7LC01012E. PMID: 29120467

H Neira & AE Herr. "Kinetic analysis of enzymes immobilized in porous film arrays." Analytical Chemistry, 2017, 9(19):10311-10320. doi: 10.1021/acs.analchem.7b02075. PMID: 28858525

P Abdel-Sayed, KA Yamauchi, R Gerver, and AE Herr. "Fabrication of an open microfluidic device for immunoblotting", Analytical Chemistry, 2017, 89(18):9643-9648. doi: 10.1021/acs.analchem.7b02406. PMID: 28825964

E Sinkala, E Sollier, C Renier, E Rosas-Canyelles, J Che, K Heirich, TA Duncombe, J Vlassakis, KA Yamauchi, H Huang, SS Jeffrey, & AE Herr. "Multiplexed western blotting of single circulating tumor cells." Nature Communications, 2017, 8:14622. doi: 10.1038/ncomms14622. PMID: 28332571

JJ Kim, E Sinkala, and AE Herr. "High-selectivity cytology via lab-on-a-disc western blotting of individual cells", Lab Chip, 2017, 17(5):855-863. doi: 10.1039/c6lc01333c. PMID: 28165521

KA Yamauchi & AE Herr. "Subcellular western blotting of single cells." Microsystems & Nanoengineering (Nature Publishing Group), 2017, 3:16079, doi:10.1038/micronano.2016.79. PMID: 29333327 | PDF

Y Pan, EK Sackmann, K Wypisniak, M Hornsby, SS Datwani, & AE Herr. "Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis." Scientific Reports, 2016, 6:39774, doi: 10.1038/srep39774

AM Tentori*, KA Yamauchi*, & AE Herr. "Detection of Isoforms Differing by a Single-charge Unit in Individual Cells." Angewandte Chemie, 2016, 26;55(40):12431-5. doi: 10.1002/anie.201606039, *Equal contributors. Corrigendum

C-C Kang, KA Yamauchi*, J Vlassakis*, E Sinkala, TA Duncombe, & AE Herr. "Single cell-resolution western blotting." Nature Protocols, 2016 11:1508-30, doi:10.1038/nprot.2016.089. PMID: 27466711 | PDF * equal contributors

MA Kapil, Y Pan, TA Duncombe, AE Herr. "Kinetic rate determination via electrophoresis along an open microchannel with varying cross-sectional area." Analytical Chemistry, 2016, 88(7):3669-76.

TA Duncombe*, C-C Kang*, S Maity, N Murthy, TM Ward, MD Pegram, & AE Herr. "Hydrogel pore-size modulation for enhanced single-cell western blotting." Advanced Materials, 2016, 28(2):327-34. *Equal contributors

J Vlassakis & AE Herr. "Effect of polymer hydration state on in-gel immunoassays." Analytical Chemistry, 2015, 87(21):11030-8.

TA Duncombe, AM Tentori, & AE Herr. "Microfluidics: reframing biological enquiry." Nature Reviews Molecular Cell Biology, 2015, (Invited Review), 16(9):554-67.

CA Kellenberger, J Sales-Lee, Y Pan, MM Gassaway, AE Herr, and MC Hammond. "A Minimalist Biosensor: Quantitation of Cyclic Di-GMP Using Conformational Change of a Riboswitch." RNA Biology, 2015, 12(11):1189-97.

R Lin, A Skandarajah, RE Gerver, HD Neira, DA Fletcher & AE Herr. "A lateral electrophoretic flow diagnostic assay." Lab Chip, 2015, 86(21):10625-32.

R Gerver & AE Herr. "Microfluidic Western blotting of low molecular mass proteins." Analytical Chemistry, 2014, 86(21):10625-32.

Y Pan, TA Duncombe, C Kellenberger, MC Hammond & AE Herr. "High-throughput electrophoretic mobility shift assays for quantitative analysis of molecular binding reactions." Analytical Chemisry, 2014, 86(20):10357-64.

C-C Kang, J-M Lin, Z Xu, S Kumar & AE Herr. "Single-cell western blotting after whole-cell imaging to assess cancer chemotherapeutic response." Analytical Chemisry, 2014, 86(20):10429-36.

M Chung*, D Kim* & AE Herr. "Polymer sieving matrices in microanalytical electrophoresis." Analyst, 2014, 139(22):5635-54.*Equal contributors (Invited Review)

AJ Hughes*, DM Spelke*, Z Xu, C-C Kang, DV Schaffer, & AE Herr. "Single-cell western blotting." Nature Methods, 2014, 11(7):749-55.*Equal contributors

AM Tentori & AE Herr. "Performance implications of chemical mobilization after microchannel isoelectric focusing." Electrophoresis, 2014, 35(10):1453-60.

MA Kapil & AE Herr. "Binding kinetic rates measured via electrophoretic band crossing in a pseudo-homogeneous format." Analytical Chemistry, 2014, 4;86(5):2601-9.

Y Pan, K Karns, & AE Herr. "Microfluidic electrophoretic mobility shift assays for quantitative biochemical analysis." Electrophoresis, 2014, 35(15):2078-90.(Invited Review)

MK Araz, AA Apori, C Salisbury, & AE Herr. "Microfluidic barcode assay for antibody-based confirmatory diagnostics." Lab on a Chip, 2013, 13(19):3910-20.

M Chung, D Kim, & AE Herr. "Microchamber Western blotting using poly-L-lysine conjugated polyacrylamide gel for blotting of SDS coated proteins." Analytical Chemistry, 2013, 85(16):7753-61.

D Kim & AE Herr. "Protein immobilization techniques for microfluidic assays." Biomicrofluidics, 2013, 7(4):41501.

AE Herr. "Disruptive by Design: A perspective on engineering in analytical chemistry." Analytical Chemistry, 2013, 85(16):7622-8.

MK Araz, AM Tentori, & AE Herr. "Microfluidic multiplexing in bioanalyses." JALA, 2013, 18(5):350-66. (Invited Review)

AM Tentori, AJ Hughes, & AE Herr. "Microchamber Integration unifies distinct separation modes for Two-Dimensional Electrophoresis." Analytical Chemistry, 2013, 85(9):4538-4545.

TA Duncombe & AE Herr. "Photopatterned free-standing polyacrylamide gels for microfluidic protein electrophoresis." Lab Chip, 2013, 13(11):2115-23.

K Karns, JM Vogan, Q Qin, S Hickey, MC Hammond & AE Herr. "Microfluidic Screening of Electrophoretic Mobility Shifts Elucidates Riboswitch Binding Function." Journal of the American Chemical Society (JACS), 2013, 135(8):3136-43.

AA Apori, M Brozynski, I El-Sayed, & AE Herr. "Microfluidic validation of diagnostic protein markers for spontaneous cerebrospinal fluid rhinorrhea." Journal of Proteome Research, 2013, 12(3):1254-65.

SQ Tia, K Brown, D Chen & AE Herr. "Protein post-translational modification analyses using immunoprobed isoelectric focusing."Analytical Chemistry, 2013, 85(5):2882-90.

C Hou & AE Herr. "Microfluidic integration of Western blotting is enabled by electrotransfer-assisted sodium dodecyl sulphate dilution." Analyst, 2013, 138(1):158-63.

AJ Hughes & AE Herr. "Microfluidic Western blotting." Proceedings of the National Academy of Sciences USA, 2012, 109(52):21450-5.

TA Duncombe & AE Herr. "Polyacrylamide gel moving boundary electrophoresis enables low-power protein analysis in a compact microdevice."Analytical Chemistry, 2012, 84(20):8740-7.

AJ Hughes*, AM Tentori*, & AE Herr. "Bistable isoelectric point photoswitching in green fluorescent proteins observed by dynamic immunoprobed isoelectric focusing." Journal of the American Chemical Society (JACS), 2012, 134(42):17582-91. *Equal contributors

AK Denisin*, K Karns*, & AE Herr. "Post-collection processing of Schirmer Strip-collected human tear fluid impacts protein content." Analyst, 2012, 137(21):5088-96. *Equal contributors

AJ Hughes, RKC Lin, DM Peehl, & AE Herr. "Microfluidic integration for automated targeted proteomic assays." Proceedings of the National Academy of Sciences USA, 2012, 109(16):5972-7.

D Kim, K Karns, S Tia, M He, & AE Herr."Electrostatic protein immobilization using charged polyacrylamide gels and cationic detergent microfluidic Western Blotting." Anal Chem, 2012, 84(5), pp 2533-40.

M He, J Novak, B Julian & AE Herr. "Membrane assisted on-line renaturation for automated microfluidic lectin blotting." JACS, 2011, 133(49):19610-3.

K Karns & AE Herr. "Human tear protein analysis enabled by an alkaline microfluidic homogeneous immunoassay." Analytical Chemistry, 2011, 83(21):8115-22.

X Chen, M Kapil, AJ Hughes & AE Herr. "A single-microchannel, multi-step assay reports protein size and immunoaffinity." Analytical Chemistry, 2011, 83(17):6573-9.

SQ Tia, M He, D Kim & AE Herr. "Multi-analyte on-chip native Western blotting." Analytical Chemistry, 2011, 83(9):3581-8.

AM Tentori & AE Herr. "Photopatterned materials in bioanalytical microfluidic technology." J. Micromech. Microeng, 2011, 21(5):54001.

A Apori & AE Herr. "Homogeneous immunosubtraction integrated with sample preparation is enabled by a microfluidic format." Analytical Chemistry, 2011, 83(7):2691-8.

JS Kinney, T Morelli, T Braun, CA Ramseier, AE Herr, JV Sugai, CE Shelburne, LA RAyburn, AK Singh, WV Giannobile. "Saliva/pathogen biomarker signatures and periodontal disease progression." Journal of Dental Research, 2011, 90(6):752-8.

M He & AE Herr. "Automated microfluidic protein immunoblotting." Nature Protocols, 2010, 5, 1844-56.[ pdf SI ]

AJ Hughes & AE Herr. "Quantitative enzyme activity determination with zeptomole sensitivity by microfluidic hradient-hel zymography." Analytical Chemistry, 2010, 82, 3803-11.[ pdf SI ]

C Hou & AE Herr. "Ultra-short separation length homogeneous electrophoretic immunoassays using on-chip discontinuous Polyacrylamide gels." Analytical Chemistry, 2010, 82, 3343-51.[ pdf ]

M He & AE Herr. "Polyacrylamide gel photopatterning enables automated protein immunoblotting in a two-dimensional microdevice." Journal of the American Chemical Society, 2010, 132, 2512-2513.[ pdf SI ]

M He & AE Herr. "Microfluidic polyacrylamide gel electrophoresis with in-situ immunoblotting for native protein analysis." Analytical Chemistry, 2009, 81, 8177-84.[ pdf ]

N Srivastava, JS Brennan, RF Renzi, M Wu, SS Branda, AK Singh, & AE Herr. "Fully-integrated microfluidic platform enables automated phosphoprofiling of macrophage response." Analytical Chemistry, 2009, 81, 3261-3269.[ pdf ]

S Tia & AE Herr. "On-chip technologies for multi-dimensional separations." Lab Chip, 2009, 9, 2524 - 2536.[ pdf ]

CA Ramseier, JS Kinney, AE Herr, T Braun, JV Sugai, CA Shelburne, LA Rayburn, HM Tran, AK Singh, WV Giannobile. "Identification of pathogen and host-response markers correlated with periodontal disease." J Periodontol, 2009, 80(3), 436-46.[ pdf ]

C Hou & AE Herr. "Clinically-relevant advances in on-chip affinity-based electrophoresis and electrochromatography." Electrophoresis, 2008, 29, 3306-3319. [ pdf ] (Invited Review)

CT Lo¹, DJ Throckmorton¹, AK Singh & AE Herr. "Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing." Lab Chip, 2008, 8, 1273-1279. ¹equal contributors [ pdf ][ RSC Chemical Biology ] Selected as a 2008 Hot Article

AE Herr, AV Hatch, DJ Throckmorton, HM Tran, JS Brennan, WV Giannobile, AK Singh. "Microfluidic immunoassays as rapid saliva-based clinical diagnostics." Proc Natl Acad Sci USA, 2007, 104(13), 5268-5273. [ pdf ][ NIH 'Research Matters' ][ NIH press release ]

AE Herr, AV Hatch, WV Giannobile, DJ Throckmorton, HM Tran, JS Brennan, AK Singh. "Integrated microfluidic platform for oral diagnostics." Ann NY Acad Sci, 'Oral Based Diagnostics, Part VI: Horizons in Oral Diagnostics', 2007, 1098, 362-374.

AV Hatch, AE Herr, DJ Throckmorton, JS Brennan, and AK Singh. "Integrated preconcentration SDS-PAGE of proteins in microchips using photopatterned cross-linked polyacrylamide gels." Analytical Chemistry, 2006, 78(14), 4976 - 4984. [ pdf ]

VC Rucker, KL Havenstrite, BA Simmons, SM Sickafoose, AE Herr, R Shediac. "Functional antibody immobilization on 3-dimensional polymeric surfaces generated by reactive ion etching." Langmuir, 2005, 21(17), 7621-7625. [ pdf ]

VC Rucker, KL Havenstrite, AE Herr. "Antibody microarrays for native toxin detection." Anal Biochem, 2005, 339(2), 262-270.

AE Herr, DJ Throckmorton, AA Davenport, AK Singh. "On-chip native gel electrophoresis-based immunoassays for tetanus antibody and toxin." Analytical Chemistry, 2005, 77(2), 585-590. [ pdf ]

AE Herr & AK Singh. "Photopolymerized cross-linked polyacrylamide gels for on-chip protein sizing." Analytical Chemistry, 2004, 76(16), 4727-33. [ pdf ]

AE Herr, JI Molho, KA Drouvalakis, JC Mikkelsen, PJ Utz, JG Santiago, TW Kenny, "On-Chip coupling of isoelectric focusing and free solution electrophoresis for Multi-Dimensional Separations," Anal Chem, 2003, 75(5), 1180-1187. [ pdf ]

F Raisi, P Belgrader, DA Borkholder, AE Herr, GJ Kintz, F Pourhamadi, MT Taylor, MA Northrup, "Microchip isoelectric focusing using a miniature scanning detection system", Electrophoresis, 2001, 22(11), 2291-2295. [ pdf ]

JI Molho, AE Herr, BP Mosier, JG Santiago, TW Kenny, RA Brennen, GB Gordon, B Mohammadi. "Optimization of turn geometries for microchip electrophoresis," Anal Chem, 2001, 73(6),1350-1360. [ pdf ]

AE Herr, JI Molho, JG Santiago, MG Mungal, TW Kenny, MG Garguilo. "Electroosmotic capillary flow with nonuniform Zeta potential." Analytical Chemistry, 2000, 72(5), 1053-1057. [ pdf ]


Conferences, book chapters & invited talks 

please see Prof. Herr's [ CV ]